Coding
Gas6

Part:BBa_K3842000:Design

Designed by: Xiaoyi Feng   Group: iGEM21_GA_State_SW_Jiaotong   (2021-10-17)


Coding sequence for human growth arrest-specific protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 946
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 946
    Illegal NotI site found at 2062
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 512
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 946
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 946
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Opitimization for pichia pastoris


Source

The sequence comes from https://www.ncbi.nlm.nih.gov/nuccore/NM_000820.4 And it was optimized by thermofisher

References